Abstract: Nuclear power plants use accident procedures for accident management, which are mainly divided into paper and digital according to the type of carrier. The accident-procedure information system is a newly developed mobile digital procedure, which provides convenience for the main control room operator to deal with the emergency, but it also brings new human factors, especially the impact on operators' workload after the accident, which must be effectively evaluated. Taking paper regulations and information system as independent variables, the influence of information system on operator's workload is discussed by using surface-EMG technique and subjective scale. The experimental results show that the regulation information system increases the workload of the operator. The main reasons are the change of the operator's behavior pattern, the higher weight of the regulation terminal, interface management tasks, software and hardware adaptation, and the operator's lack of expertise. This research can provide reference for the design and optimization of information system and operator training.