Abstract: Using the queuing theory method, the service system of large and small lockers of intelligent express cabinets were abstracted into a M/M/n/n loss queuing system respectively, and the income model of express cabinet supplier was constructed. Through the case numerical experiment, the pricing strategy of the lockers under the situation of the supplier's profit maximization was explored and the influences of relevant parameters on the supplier's maximum profit and the optimal price of the lockers were discussed. The results show that, if the lockers are reasonably set to match the express delivery requirements in a service area and a certain amount of express cabinet advertising revenue is guaranteed, the current locker price of 0.3 ~ 0.6 yuan/time can be appropriately reduced, and the users need not or should not pay the suppliers any fees for normal use of lockers, which can ensure reasonable profits for suppliers and couriers using express cabinets, and increase their willingness to use express cabinets.