Abstract: In view of the cold chain logistic distribution routing optimization, based on the concept of green supply chain and the characteristics of the cold chain logistics distribution process, the fixed cost of the vehicle, the fuel cost of the distribution process, the freight loss cost of the distribution process and the carbon emission cost were analyzed, and a mathematical model was constructed with the minimum sum of the above costs as the optimization target. The global artificial fish swarm algorithm was applied to the model. Finally, the Matlab software was used to solve the specific case and the total cost is 4,456 yuan. The result of the case shows that the mathematical model and global artificial fish swarm algorithm have certain theoretical guidance and reference value for solving such problems.